Download free transfection by electroporation of raw 264.7 macrophages

Transfection efficiency of GeneX Plus Reagent on RAW mouse leukemic monocyte/macrophage cells (×10). Cells were transfected with EF1α -eGFP empty vector at μg DNA with μL of reagent () in Opti- MEM I Reduced Serum Medium per well of a well plate. Download Free PDF. Download Free PDF. Exosome-mediated delivery of functionally active miRNA inhibitor to macrophages. Nanomedicine: nanotechnology, biology, and medicine, Shashi Bala. Download PDF. Download Full PDF Package. This paper. A short summary of this paper. Do you have a protocol for transfection of macrophage cell lines (JA1 and RAW ) with siRNA? Yes, please follow the protocol "Transfection of Macrophage Cell Lines, Including JA1 and RAW , with siRNA in Well Plates" in the HiPerFect Transfection Reagent Handbook.

Protocol Transfection by Electroporation of RAW Macrophages. Stephen T. Smale; Cold Spring Harb Protoc; ; doi: /bltadwin.ru Registration is free. Download to citation manager; Permissions Personal Folder. Save to Personal Folders. Download Citation | Transfection by Electroporation of RAW Macrophages | INTRODUCTION In electroporation-based transfection methods, the DNA and cells are mixed in an empirically defined. Most of the existing knowledge about monocytes and macrophages comes from either mouse models or cell lines such as murine RAW and human THP-1 Neither of these cells represents human.

Thompson CD, Frazier-Jessen MR, Rawat R, Nordan RP, Brown RT. Evaluation of methods for transient transfection of a murine macrophage cell line RAW Biotechniques. ; 27 (4)– [Google Scholar] Kim T, Eberwine J. Mammalian cell transfection: the present and the future. Analytical and Bioanalytical Chemistry. On pages , you have references for siRNA transfection into RAW macrophages, and on page 4 for plasmid transfection. If you want trial kits for free, just write me at bltadwin.ruere@lipocalyx. Electroporation is an attractive method for transfection because it is more rapid and involves fewer manipulations than other methods and can be used for many types of adherent and nonadherent cells. This protocol describes transfection by electroporation of RAW macrophages.